Add to ORKGPathogen genotyping of polyclonal infections is limited by 2 major drawbacks: (1) how to establish whether multiple mu-tations detected in 1 gene belong to the same clone and (2) how to evaluate the proportion of different genotypes in a given sample. For drug-resistance genotyping in Plasmodium falciparum malaria, we address these problems by using a fluorogenic assay that combines fluorescence-resonance en-ergy transfer, between fluorophores present on a probe and a polymerase–chain-reaction primer, and a melt-curve anal-ysis. We demonstrate that this tool allows a more accurate insight into the P. falciparum populations present in complex biological samples. Pathogen genotyping has become essential for the monitoring and control of infec...
Abstract. Individuals living in malaria endemic areas are often infected with multiple parasite clon...
Naturally acquired blood-stage infections of the malaria parasite Plasmodium falciparum typically ha...
Abstract Background Single nucleotide polymorphism (SNP) genotyping provides the means to develop a ...
The diversity in the Plasmodium falciparum genome can be used to explore parasite population dynamic...
The diversity in the Plasmodium falciparum genome can be used to explore parasite population dynamic...
Parasite genotyping by the polymerase chain reaction was used to distinguish recrudescent from newly...
Abstract Genetic diversity of malaria parasites represents a major issue in understanding several as...
Genetic diversity of malaria parasites represents a major issue in understanding several aspects of ...
Despite efforts to reduce malaria morbidity andmortality, drug-resistant parasites continue to evade...
Abstract. Parasite genotyping by the polymerase chain reaction was used to distinguish recrudescent ...
Simultaneous infection with multiple pathogens of the same species occurs with HIV, hepatitis C, Eps...
Current methods used to genotype point mutations in Plasmodium falciparum genes involved in resistan...
Background: Single nucleotide polymorphism (SNP) genotyping provides the means to develop a pract...
Abstract. Studying drug resistance in Plasmodium falciparum requires accurate measurement of parasit...
Differentiation between gametocyte-producing Plasmodium falciparum clones depends on both high level...
Abstract. Individuals living in malaria endemic areas are often infected with multiple parasite clon...
Naturally acquired blood-stage infections of the malaria parasite Plasmodium falciparum typically ha...
Abstract Background Single nucleotide polymorphism (SNP) genotyping provides the means to develop a ...
The diversity in the Plasmodium falciparum genome can be used to explore parasite population dynamic...
The diversity in the Plasmodium falciparum genome can be used to explore parasite population dynamic...
Parasite genotyping by the polymerase chain reaction was used to distinguish recrudescent from newly...
Abstract Genetic diversity of malaria parasites represents a major issue in understanding several as...
Genetic diversity of malaria parasites represents a major issue in understanding several aspects of ...
Despite efforts to reduce malaria morbidity andmortality, drug-resistant parasites continue to evade...
Abstract. Parasite genotyping by the polymerase chain reaction was used to distinguish recrudescent ...
Simultaneous infection with multiple pathogens of the same species occurs with HIV, hepatitis C, Eps...
Current methods used to genotype point mutations in Plasmodium falciparum genes involved in resistan...
Background: Single nucleotide polymorphism (SNP) genotyping provides the means to develop a pract...
Abstract. Studying drug resistance in Plasmodium falciparum requires accurate measurement of parasit...
Differentiation between gametocyte-producing Plasmodium falciparum clones depends on both high level...
Abstract. Individuals living in malaria endemic areas are often infected with multiple parasite clon...
Naturally acquired blood-stage infections of the malaria parasite Plasmodium falciparum typically ha...
Abstract Background Single nucleotide polymorphism (SNP) genotyping provides the means to develop a ...