Add to ORKGThe rat is an important animal model in biomedical research, but gene targeting technology is not established for this species. Therefore, we aimed to produce transgenic knockdown rats using shRNA technology and pronuclear microinjection. To this purpose, we employed a tetracycline-inducible shRNA expression system targeting the insulin receptor (IR). Doxycycline (DOX) treatment of the resulting transgenic rats led to a dose-dependent and reversible increase in blood glucose caused by ubiquitous inhibition of IR expression and signalling. We could neither detect an interferon response nor disturbances in microRNA processing after DOX treatment excluding toxic effects of shRNA expression. Low dose DOX treatment induced a chronic state of dia...
This Major Qualifying Project (MQP) addresses an important step in the development of a cure for dia...
BACKGROUND: Previous studies demonstrating the efficacy of insulin gene therapy have mostly involved...
To develop transgenic lines for conditional expression of desired genes in rats, we generated severa...
The rat is an important animal model in biomedical research, but gene targeting technology is not es...
The rat is an important animal model in biomedical research, but gene targeting technology is not es...
The rat is an important animal model in biomedical research, but gene targeting technology is not es...
The rat is an important animal model in biomedical research, but gene targeting technology is not es...
All in-text references underlined in blue are linked to publications on ResearchGate, letting you ac...
<p>The transgene construct, pTet-shIR (A), contains two expression cassettes: One expresses shRNA ag...
The purpose of this study was to determine whether the treatment with recombinant plasmid consisted ...
Insulin gene therapy in clinical medicine is currently hampered by the inability to regulate insulin...
Context Type 1 diabetes mellitus is a T cell mediated autoimmune disease with no known methods of pr...
International audienceInsulin receptor (IR)-deficient mice develop severe diabetes mellitus, diabeti...
<p>(A) Tet29 rats were treated with doxycycline (DOX) as described in <a href="http://www.plosone.or...
The lack of germline-competent ES cells makes gene inactivation through homologous recombination imp...
This Major Qualifying Project (MQP) addresses an important step in the development of a cure for dia...
BACKGROUND: Previous studies demonstrating the efficacy of insulin gene therapy have mostly involved...
To develop transgenic lines for conditional expression of desired genes in rats, we generated severa...
The rat is an important animal model in biomedical research, but gene targeting technology is not es...
The rat is an important animal model in biomedical research, but gene targeting technology is not es...
The rat is an important animal model in biomedical research, but gene targeting technology is not es...
The rat is an important animal model in biomedical research, but gene targeting technology is not es...
All in-text references underlined in blue are linked to publications on ResearchGate, letting you ac...
<p>The transgene construct, pTet-shIR (A), contains two expression cassettes: One expresses shRNA ag...
The purpose of this study was to determine whether the treatment with recombinant plasmid consisted ...
Insulin gene therapy in clinical medicine is currently hampered by the inability to regulate insulin...
Context Type 1 diabetes mellitus is a T cell mediated autoimmune disease with no known methods of pr...
International audienceInsulin receptor (IR)-deficient mice develop severe diabetes mellitus, diabeti...
<p>(A) Tet29 rats were treated with doxycycline (DOX) as described in <a href="http://www.plosone.or...
The lack of germline-competent ES cells makes gene inactivation through homologous recombination imp...
This Major Qualifying Project (MQP) addresses an important step in the development of a cure for dia...
BACKGROUND: Previous studies demonstrating the efficacy of insulin gene therapy have mostly involved...
To develop transgenic lines for conditional expression of desired genes in rats, we generated severa...