Resume

Despite the fact that PCR is a powerful tool in modern biology and medicine, its quantitative model describing product accumulation at later stages of amplification is not developed yet. Results: Kinetics of standard amplification is treated as recurrent scheme with a variable amplification factor H(n) introduced. At each cycle number n, the H(n) is obtained from solving a system of equations of chemical kinetics, which takes into account the reassociation and destruction of PCR product. The amplification limit is calculated as a fixed stable point H=1 of the transformation. A good agreement has been found between the results obtained under the proposed model and the known experimental data. The model can be straightforwardly generalized for use in more realistic PCR computer simulation