A New Model of Laryngitis: Neuropeptide,

Objectives/Hypothesis: To develop and character-ize a new model of laryngeal inflammation by analyzing the presence of neurogenic peptides and expression of cyclooxygenases (COX) and cytokines in the mucosa. Study Design: Laryngitis induced by nasogastric intubation (NGI) was evaluated by histopathologic changes of the mucosa, alterations in calcitonin gene re-lated peptide (CGRP) and substance P (SP) neuropep-tides in sensory fibers, and COX-1,2, and cytokine (inter-leukin [IL]-1, IL-6, IL-10, tumor necrosis factor [TNF]-) expression in the laryngeal mucosa. Methods: Rats submitted to NGI for 1 to 5 weeks were compared with controls. Laryngeal sections were immunostained for stereologic analysis of SP and CGRP fiber density and number of mucosal cells expressin