Macrophage Interleukini a,-1 , and Tumor Necrosis Factor Production During the Peritoneal Exudative Response

The present study examined changes in Iipopolysaccharide (LPS)-Induced Interleukln 1 (IL-i) and tumor necrosis factor (TNF) production by murine peritoneal macrophages during the chronic exudative response to Freund’s complete adjuvant (CFA). Macro-phages were Isolated by peritoneal lavage and adherence at Intervals over a 32 day period following l.p. Injection of CFA. Optimal culture condItions for IL-i and TNF pro-duction were predetermined, and it was found that IL-i production was profoundly im-paired at densities of above 150 cells/mm2, whereas TNF sythesis was more resistant to density effects. Using optimal condItions, we observed a sequential appearance of monoklnes. On day 0 there was minimal IL-i productIon and no detectable TNF produc-tion. By days 4-7, IL-i production reached maxImum levels with a steady decline to baseline by day 32. TNF productIon steadily increased after day 2, reached maximal levels by days 16-20, and then partly declined by day 32. These findings were supported by kinetIc analyses at specIfied days. When related to exudative events, It appeared that maximal IL-I was associated with the recruitment stage of the reactIon, whereas TNF production was associated with the established exudate. Immunohlstochemical analysi