Add to ORKGWe describe a rapid and simple assay for DNA damage and repair in mammalian cells, based on the partial unwinding of nicked DNA in alkali, and involving transfer of this DNA to nitrocellulose and the digestion of single-stranded DNA with Sj nuclease, all steps taking place on tissue-culture chamber slides. Direct breakage of DNA by ionizing radiation has been examined, and we have developed a standard procedure for measuring enzymic breakage of DNA as an index of excision-repair capacity following ultraviolet irradiation. We report a wide range of repair capacities among various hamster and human cell lines, with considerable overlap between the two species. Hybrids between hamster and human cells tend to display repair activity characteris...
Illumination of Chinese hamster cells with fluorescent light after 5-bromodeoxyuridine incorporation...
2To whom correspondence should be addressed Exposure of cells to ionizing radiation gives rise to DN...
DNA damage in mammalian chromatin in vitro and in cultured mammalian cells including human cells was...
Nucleotide excision repair (NER) is one of the mechanisms of how to repair DNA damaged by the extern...
The kinetics and dose dependence were determined both for the repair synthesis and for the induction...
The occurrence of DNA repair in UV- (254 nm) and X-irradiated normal human diploid fibroblasts maint...
It generally accepted that the critical target of ionizing radiation in cellular reproductive death ...
When Chinese hamster cells are lysed on top of an alkaline sucrose gradient, in time a fairly discre...
The incidence of pyrimidine dimer formation and the kinetics of DNA repair in African green monkey k...
In this paper we examine DNA repair in the mouse. Using stringently applied inhibitors of DNA synthe...
We have developed a new assay which allows us to monitor the rates of repair of potentially lethal d...
Cells defective in one or more aspects of repair are killed and often mutagenized more readily than ...
Postreplication repair in synchronous Chinese hamster cells was determined after split doses of ultr...
Two mammalian cell lines, Chinese hamster ovary (CHO) which can recover colony-forming ability betwe...
The studies of DNA damage in living cells in vitro and in vivo were continued. A variety of systems ...
Illumination of Chinese hamster cells with fluorescent light after 5-bromodeoxyuridine incorporation...
2To whom correspondence should be addressed Exposure of cells to ionizing radiation gives rise to DN...
DNA damage in mammalian chromatin in vitro and in cultured mammalian cells including human cells was...
Nucleotide excision repair (NER) is one of the mechanisms of how to repair DNA damaged by the extern...
The kinetics and dose dependence were determined both for the repair synthesis and for the induction...
The occurrence of DNA repair in UV- (254 nm) and X-irradiated normal human diploid fibroblasts maint...
It generally accepted that the critical target of ionizing radiation in cellular reproductive death ...
When Chinese hamster cells are lysed on top of an alkaline sucrose gradient, in time a fairly discre...
The incidence of pyrimidine dimer formation and the kinetics of DNA repair in African green monkey k...
In this paper we examine DNA repair in the mouse. Using stringently applied inhibitors of DNA synthe...
We have developed a new assay which allows us to monitor the rates of repair of potentially lethal d...
Cells defective in one or more aspects of repair are killed and often mutagenized more readily than ...
Postreplication repair in synchronous Chinese hamster cells was determined after split doses of ultr...
Two mammalian cell lines, Chinese hamster ovary (CHO) which can recover colony-forming ability betwe...
The studies of DNA damage in living cells in vitro and in vivo were continued. A variety of systems ...
Illumination of Chinese hamster cells with fluorescent light after 5-bromodeoxyuridine incorporation...
2To whom correspondence should be addressed Exposure of cells to ionizing radiation gives rise to DN...
DNA damage in mammalian chromatin in vitro and in cultured mammalian cells including human cells was...