INTERACTION OF SEVERAL TRADITIONAL MEDICINE PREPARATIONS WITH AMINOPYRINE IN RAT HEPATOCYTES

Extractum and King To Nin Jiom Pei Pa Kao) traditional medicine preparations aminopyrine in rat liver hepatocytes. Aminopyrine N-demethylase activity was determined by measuring the quantity of formaldehyde formed. Results indicate that Faizol Tonik a and Air Jamu Pak Tamu increase the activity of aminopyrine N-demethylase significantly (P<0.05) whereas Air Gamat Asli Langkawi produces no significant change in the enzymatic activity when compared to control. With the Chinese traditional medicine preparations, all three preparations investigated increase the activity of aminopyrine N-demethylase significantly (P<0.05). Thus, several of the traditional medicine preparations tested were found to affect the metabolism of aminopyrine in-vitro and whether the biovailability of aminopyrine in-vivo can be similarly affected by these preparations remains to be investigate