Proceedings ofthe 9th International Symposium on Buckwheat, Prague 2004 Identification and Genetics of Buckwheat (Fagopyrum) Seed Storage Protein

Total seed protein of 22 cross-pollinated common buckwheat (Fagopyrum esculentum Moench) cultivars and two self-fertile, wild buckwheat species, F. homotropicum and F. esculentum ssp. ancestrale within a molecular weight range of 20-57 kDa were analysed by Sodium dodecyl sulphate polyacrylamide- gel electrophoresis (SDS-PAGE). The electropherogramm of F. homotropicum indicates a homogenous pattern, whereas the common buckwheat showed high intravarietal polymorphism. A scheme has been developed to differentiate all the cultivars analysed. The l3S predominant seed globulins of buckwheat were found in two main groups being expressed in the cultivars and in hybrid progenies. Genetic analysis of the mode of inheritance was conducted employing two F3 populations involving (1) a self-fertile cv. Sumchanka and F. homotropicum and (2) a self-fertile cultivar Kora and F. esculentum ssp. ancestrale. Results from both hybridized populations indicate a codominant monogenic mode of inheritance of the seed globulin