LOCALIZED CUTANEOUS LEISHMANIASIS DUE TO LEISHMANIA DONOVANI AND LEISHMANIA TROPICA: PRELIMINARY FINDINGS OF THE STUDY OF 161 NEW CASES FROM A NEW ENDEMIC FOCUS IN HIMACHAL PRADESH, INDIA

Abstract. Localized cutaneous leishmaniasis (LCL) in India is due mostly to Leishmania tropica. It is mainly endemic in the deserts of Rajasthan. Recently, Himachal Pradesh has been identified as a new endemic focus for the disease. In the last few years, the number of new cases has been increasing almost to epidemic proportions. This report presents the preliminary findings of clinico-epidemiologic and investigative results of 161 new localized cases of LCL seen between May 2001 and December 2003. The study populaton was composed of 80 males and 81 females between 10 months and 75 years of age. All were indigenous to the sub-alpine valley along the Satluj River in the mountainous region of the Kinnaur District (altitude 700−2,900 meters). Most patients were seen from April to September and had 1−8 lesions (duration 1−6 months) that involved mainly the face. Tissue smears were positive for amastigotes in 37 % and histopathology showed non-caseating epitheloid cell granuloma in 77 % of the cases. Analysis by polymerase chain reaction−restriction fragment length polymorphism (PCR-RFLP) of the ribosomal gene region of 10 biopsy specimens showed amplicons indistinguishable from L. donovani in eight cases and L. tropica in two cases. Leishmania was cultured on modified Nicole-Novy-McNeal (NNN) medium containing RPMI 1640 medium and heat-inactivated fetal bovine serum from 13 of 38 biopsy samples. Three of these isolated strains were identified as L. donovani while a fourth was L. tropica by PCR-RFLP of the ribosomal internal transcribed spacer region. One strain had a gp63 sequence identical to that of east African strains. Another strain had a unique gp63 sequence that has not been found in L. donovan