Integrating Probe-Level Expression Changes across Generations of Affymetrix Arrays

There is an urgent need for bioinformatic methods that allow integrative analysis of multiple microarray datasets.While previousstudieshavemainlyconcen-trated on reproducibility of gene expression levels within or between different platforms, we propose a novel meta-analytic method that takes into account the vast amount of available probe-level information to combine the expression changes across different studies. We first show that the comparability of relative expression changes and the consistency of differentially expressed genes between different Affymetrix array generations can be considerably improved by determining the expression changes at the probe-level and by considering the latest information on probe-level sequence matching instead of the probe annotations provided by the manufacturer. With the improved probe-level expres-sion change estimates, data from different genera-tions of Affymetrix arrays can be combined more effectively. This will allow for the full exploitation of existing results when designing and analyzing new experiments