The granulin gene region of Cryptophlebia leucotreta granulosis virus: sequence analysis and phylogenetic onsiderations

The nucleotide sequence of a 2.2 kb genome region of the baculovirus Cryptophlebia leueotreta granulosis virus (CIGV) encompassing the granulin gene and a second open reading frame, designated ORF909, was deter-mined. The putative granulin ORF comprises 744 nucleotides encoding a polypeptide with a predicted Mr of 29.3K. The 5 ' leader of the granulin gene contains a canonical late baculovirus promoter (ATAAG) and differs only in two nucleotide positions from the sequence of Cydia pomonella granulosis virus (CpGV). Sequence comparison with the granulin genes of dif-ferent granulosis viruses indicated a very close re-lationship between C1GV and CpGV of about 96% amino acid identity. ORF909 is 909 nucleotides in length and encodes a potential protein of M r 36.2K. I