2Department of Molecular and Cellular Biochemistry

DB-cAMP, dibutyryl cyclic AMP; DB-cGMP, dibutyryl cyclic GMP; eNOS, endothelial nitric oxide synthase; HUVECs, human umbilical vein endothelial cells; NMA, NG-monomethyl-L-arginine; NO, nitric oxide; ODQ, 1H-(1,2,4)-oxadiazole[4,3-a]quinoxalon-1-one; PGE2, prostaglandin E2; PI3K, phosphatidylinositol 3-kinase; PKA, cAMP-dependent protein kinase; PKI, myristoylated protein kinase A inhibitor amide 14-22; Wort, wortmannin Prostaglandin E2 (PGE2), a major product of cyclooxygenase, has been implicated in modulating angiogenesis, vascular function, and inflammatory processes, but the underlying mechanism is not clearly elucidated. We here investigated the molecular mechanism by which PGE2 regulates angiogenesis. Treatment of human umbilical vei