Multicomponent Analysis for Alkaline Phosphatase lsoenzyme Determination by Multiple Linear Regression

may be determined by multicomponent analysis of the enzyme activities in the presence of multiple inhibitors.To determine inhibition coefficients of the isoenzymes, we used multiple linear regression analysis to compare alka-line phosphatase activities in the presence of known in-hibitors with electrophoretically determined isoenzyme activities in plasma and serum samples. All possible Com-binations of exactly determined and overdetermined lin-ear systems of inhibitorswere ranked according to their prediction error to select an optimum set. The best mul-ticomponent system for prediction included the use of levamisole, phenylalanine, and heat inhibitionat 56#{176}Cand 65#{176}Cto determine bone, hepatic, intestinal, and placental isoenzymes. Consideration of the hepatic isoenzyme as liverand macromolecular fractions resulted in significantl