Identification of Pro-MMP-7 as a Serum Marker for Renal Cell Carcinoma by Use of Proteomic Analysis

marker is known for detection of asymptomatic disease in selected populations or for prognostic purposes or treatment monitoring. We identified immunogenic proteins as tumormarkers for RCC by combining con-ventional proteome analysis with serological screening, andwe investigated the diagnostic clinical value of such markers in serum. METHODS: We studied the immunogenic protein ex-pression profile of CAL 54, a human RCC cell line, by 2-dimensional electrophoresis combined with immu-noblotting using sera from healthy donors compared with RCC patients.We developed a homogeneous, flu-orescent, dual-monoclonal immunoassay for metallo-proteinase 7 (MMP-7) and used it to measure MMP-7 in sera from 30 healthy donors, 30 RCC patients, an