Correlation Between the Level of Cytogenetic Aberrations in Cultured Human Lymphocytes

To answer whether the age-related accumulation of chromosomal damage differs in men and women, and whether the aberration level in centenarians is proportional to their age, cytogenetic aberrations in dividing cells were analyzed. G-band karyotyping of mitotic spreads from lymphocytes was performed in 52 Polish centenarians and 71 controls (aged 21–78). Statistical evaluation was performed using nonparametric tests and regression analysis. The average level of all chromosomal aberrations was comparable in centenarians of both genders, but the age-related increase in chromosomal damage occurred faster in women than in men. Aging in both genders was marked by the increasing level of all aberrations rather than by chromosome-specific changes; the loss of X chromosome was the leading contributor in women. The age-related increase in the level of chromosomal damage reflected accumulation of dividing cells with a small number of aberrations. Individuals who survive to the extreme old age appear to accu-mulate aberrations at the slower rate. AGING is a process that occurs in all members ofa species. Typically conceived as characterizing a postreproductive period of life, this process in fact affects organisms of any age. It is the rate and accumulation o