Multi-nanopore force spectroscopy for DNA analysis

ABSTRACT The need for low-cost DNA sequence detection in clinical applications is driving development of new techno-logies. We demonstrate a method for detection of mutations in a DNA sequence purely by electronic means, and without need for fluorescent labeling. Our method uses an array of nanopores to perform synchronized single-molecule force spectroscopy measurements over many molecules in parallel, yielding detailed information on the kinetics of hundreds of molecule disso-ciations in a single measurement