Comparison of Full Versus Partial Metabolic Labeling for Quantitative Proteomics Analysis

In recent years a variety of quantitative proteomics tech-niques have been developed, allowing characterization of changes in protein abundance in a variety of organisms under various biological conditions. Because it allows ex-cellent control for error at all steps in sample preparation and analysis, full metabolic labeling using 15N has emerged as an important strategy for quantitative proteomics, having been applied in a variety of organisms from yeast to Arabi-dopsis and even rats. However, challenges associated with complete replacement of 14N with 15N can make its appli-cation in many complex eukaryotic systems impractical on a routine basis. Extending a concept proposed b