THE ROLES OF COMPLEMENT FACTOR C5a AND CINC-1 IN GLUCOSE TRANSPORT, ULTRAFILTRATION, AND NEUTROPHIL RECRUITMENT DURING PERITONEAL DIALYSIS

¤ Background: In a recent experimental study, we showed that low molecular weight heparin improved ultrafiltration and blocked complement activation and coagulation in a single peritoneal dialysis (PD) dwell. ¤ Objective: The aim of the present study was to evaluate the possible contribution of the complement factor C5a and the potential interactions between C5a, the coagulation sys-tem, and cytokines of the interleukin (IL)-8 family (cytokine-induced neutrophil chemoattractant; CINC-1). ¤ Methods: Nonuremic rats were exposed through an in-dwelling catheter to a single dose of 20 mL glucose- (2.5%) based filter-sterilized PD fluid, with or without the addi-tion of anti-rat C5 antibody. The dwell fluid was analyzed 2 and 4 hours later concerning activation of the coagula-tion cascades, neutrophil recruitment, ultrafiltration vol