COSMETIC Influences of Centrifugation on Cells and Tissues in Liposuction Aspirates: Optimized

Background: Although injective autologous fat transplantation is one of the most attractive options for soft-tissue augmentation, problems such as unpre-dictability and fibrosis resulting from fat necrosis limit its universal acceptance. Centrifugation is one of most common methods for overcoming these difficul-ties. This study was performed to investigate quantitatively the effects of cen-trifugation on liposuction aspirates to optimize centrifugal conditions for fat transplantation and isolation of adipose-derived stem cells. Methods: Liposuction aspirates, obtained from eight healthy female donors, were either not centrifuged or centrifuged at 400, 700, 1200, 3000, or 4200 g for 3 minutes. The volumes of the oil, adipose, and fluid portions and numbers of blood cells and adipose-derived cells in each portion were examined. The processed adipose tissues (1ml) were injected into athymicmice, and grafts were harvested and weighed at 4 weeks. Morphologic alterations were observed using light and scanning electron microscopy. Results: Centrifugation concentrated adipose tissues and adipose-derived ste