Carbonic anhydrase, an enzyme found in red blood cells, catalyses the rever-sible hydration of carbon dioxide: CO2 + H20 ±H + HCO. We have used measurements of the '3C n.m.r. linewidths of 13C02 and H'3C0 in solutions of the native human B enzyme to obtain the kinetic parameters that describe the enzymatic activity. The measurements, made under thermal equilibrium conditions and with no added buffer, were taken as a function of pH and substrate concentration at 25°C, and at pH 7.7 over a range of tempera-tures. The kinetic results, which compare well with published results obtained by stopped-flow methods in buffered solutions, are discussed in terms of the several models that have been suggested to describe the enzymatic mechanis...
Carbon dioxide absorption into carbonate solutions promoted by the enzyme carbonic anhydrase (CA, E....
The selection of a biocatalyst for the development of a biomimetic CCS processes has several constra...
The rate of carbon dioxide (CO<sub>2</sub>) absorption by monoethanol amine (MEA), diethanol amine (...
A 13C NMR saturation transfer technique was used to measure equilibrium reaction rates for the hydra...
Silverman et al. (1979. J. Am. Chem. Soc. 101:6734–6740) have reported measurements of the loss of 1...
We have measured the paramagnetic contribution to the magnetic relaxation rate of solvent protons in...
In the framework of research on post-combustion CCS processes, the will to develop eco-friendly proc...
The most recent challenge to reduce greenhouse gas emissions is the development of Carbon Capture an...
In the present work the absorption of carbon dioxide in 1000 mol m(-3) N-methyldiethanolamine (MDEA)...
A potentiometric method capable of determining carbonic anhydrase (CA) activity in vitro and based o...
In present work the absorption of carbon dioxide in aqueous N-methyldiethanolamine (MDEA) solutions ...
AbstractA CO2 hydration activity for Mn(II) human carbonic anhydrase B (MnHCAB) of 7% of the activit...
The role of structure and dynamics of an enzyme has been investigated at three different stages of i...
In native carbonic anhydrase the coordinated zinc ion, which is part of the active site region, may ...
Carbon dioxide absorption into carbonate solutions promoted by the enzyme carbonic anhydrase (CA, E....
The selection of a biocatalyst for the development of a biomimetic CCS processes has several constra...
The rate of carbon dioxide (CO<sub>2</sub>) absorption by monoethanol amine (MEA), diethanol amine (...
A 13C NMR saturation transfer technique was used to measure equilibrium reaction rates for the hydra...
Silverman et al. (1979. J. Am. Chem. Soc. 101:6734–6740) have reported measurements of the loss of 1...
We have measured the paramagnetic contribution to the magnetic relaxation rate of solvent protons in...
In the framework of research on post-combustion CCS processes, the will to develop eco-friendly proc...
The most recent challenge to reduce greenhouse gas emissions is the development of Carbon Capture an...
In the present work the absorption of carbon dioxide in 1000 mol m(-3) N-methyldiethanolamine (MDEA)...
A potentiometric method capable of determining carbonic anhydrase (CA) activity in vitro and based o...
In present work the absorption of carbon dioxide in aqueous N-methyldiethanolamine (MDEA) solutions ...
AbstractA CO2 hydration activity for Mn(II) human carbonic anhydrase B (MnHCAB) of 7% of the activit...
The role of structure and dynamics of an enzyme has been investigated at three different stages of i...
In native carbonic anhydrase the coordinated zinc ion, which is part of the active site region, may ...
Carbon dioxide absorption into carbonate solutions promoted by the enzyme carbonic anhydrase (CA, E....
The selection of a biocatalyst for the development of a biomimetic CCS processes has several constra...
The rate of carbon dioxide (CO<sub>2</sub>) absorption by monoethanol amine (MEA), diethanol amine (...
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