2D CORRELATION SPECTROSCOPY AND ITS APPLICATION IN VIBRATIONAL SPECTROSCOPY USING MATLAB

Two dimensional correlation spectroscopy is a powerfull tool for spectral analysis. It is able to reveal correlations between spectral changes and to deconvolve overlapping peaks. It is easily applicable in a study of biomolecules. Here presented program was created for easy accessibility of all necessary operations. Presented algorithm was deeply tested on regular spectra as well as on a spectra of vibrational optical activity. Basic mathematics needed for understanding and performing two-dimensional correlation spectroscopy was also presented. 1 Two dimensional correlation spectroscopy Two dimensional correlation spectroscopy (2DCoS) originated in a NMR spectroscopy. There were efforts to use it in different methods of optical spectroscopy since its development. The main reason is that 2DCos has an ability to determine an order of bands and also has superior deconvolution abilities. This effort has run into troubles with a time scale because very short light pulses are needed for excitation. That wasn’t possible until construction of femtosecond lasers and therefore existed an effort for creating a generalised form of 2DCoS. Practical us