Add to ORKGXenopus oocytes and eggs are popular models for studying the developmental and cel-lular mechanisms of RNA localization, axis specification and establishment, and nuclear envelope assembly/disassembly. However, their large size and opacity hamper application of many techniques commonly used for studying cell structure and organization, including immunofluorescence and other techniques of fluorescence microscopy. In this chapter, we describe techniques and procedures that we have used to preserve, stain, and view the cytoskeleton in Xenopus oocytes and eggs by confocal immunofluorescence microscopy. Key Words: Actin; antibodies; eggs; confocal microscopy; immunofluorescence; intermediate filaments; microtubules; oocytes; Xenopus
At the beginning of diplotene, the oocyte of Xenopus laevis is a cell of about 10–20 microns destin...
At the beginning of diplotene, the oocyte of Xenopus laevis is a cell of about 10–20 microns destin...
At the beginning of diplotene, the oocyte of Xenopus laevis is a cell of about 10–20 microns destin...
International audienceThe majority of oocyte functions involves and depends on the cytoskeletal elem...
International audienceThe majority of oocyte functions involves and depends on the cytoskeletal elem...
Summary Historically, much of our understanding of actin filaments, microtubules and intermediate fi...
In this study we report an atomic force microscopy (AFM) investigation of the actin cortical cytosk...
Experiments using cytoplasmic extracts prepared from Xenopus eggs have made important contributions ...
This protocol details methods for the isolation of oocyte nuclear envelopes (NEs) from the African c...
This protocol details methods for the isolation of oocyte nuclear envelopes (NEs) from the African c...
This protocol details methods for the isolation of oocyte nuclear envelopes (NEs) from the African c...
Concanavalin A (ConA) binding to Xenopus oocytes was studied by fluorescent and electron microscopy....
International audienceThe microtubule cytoskeleton is a plastic network of polarized cables. These p...
Self-organization of and by the cytoskeleton is central to the biology of the cell. Since their intr...
International audienceSelf-organization of and by the cytoskeleton is central to the biology of the ...
At the beginning of diplotene, the oocyte of Xenopus laevis is a cell of about 10–20 microns destin...
At the beginning of diplotene, the oocyte of Xenopus laevis is a cell of about 10–20 microns destin...
At the beginning of diplotene, the oocyte of Xenopus laevis is a cell of about 10–20 microns destin...
International audienceThe majority of oocyte functions involves and depends on the cytoskeletal elem...
International audienceThe majority of oocyte functions involves and depends on the cytoskeletal elem...
Summary Historically, much of our understanding of actin filaments, microtubules and intermediate fi...
In this study we report an atomic force microscopy (AFM) investigation of the actin cortical cytosk...
Experiments using cytoplasmic extracts prepared from Xenopus eggs have made important contributions ...
This protocol details methods for the isolation of oocyte nuclear envelopes (NEs) from the African c...
This protocol details methods for the isolation of oocyte nuclear envelopes (NEs) from the African c...
This protocol details methods for the isolation of oocyte nuclear envelopes (NEs) from the African c...
Concanavalin A (ConA) binding to Xenopus oocytes was studied by fluorescent and electron microscopy....
International audienceThe microtubule cytoskeleton is a plastic network of polarized cables. These p...
Self-organization of and by the cytoskeleton is central to the biology of the cell. Since their intr...
International audienceSelf-organization of and by the cytoskeleton is central to the biology of the ...
At the beginning of diplotene, the oocyte of Xenopus laevis is a cell of about 10–20 microns destin...
At the beginning of diplotene, the oocyte of Xenopus laevis is a cell of about 10–20 microns destin...
At the beginning of diplotene, the oocyte of Xenopus laevis is a cell of about 10–20 microns destin...