Evaluation of an Automated Glucose-Oxidase Procedure

We evaluated a slight modification of the automated oxi-dase/peroxidase method for glucose determination in blood with use of 4-aminophenazone [J. Clln. PathoL 22, 246 (1969)1. Nonsugar reducing substances did not interfere, except for ascorbic acid in very high concen-trations. Lipemic sera gave slightly lower values. Values for reproducibility, specificity, and sensitivity were clos-er to such values for the hexokinase method than was the case for some other commonly used methods. AddftlonalKeyphrase: intermethod comparison A number of mechanized methods for determining blood glucose have been developed. Some of these, such as the hexokinase method, are very specific, but their relatively high cost usually precludes their routine use. We have used various colorimetric methods such as glu-cose oxidase/peroxidase (“GOD-Perid”; Boehringer Mannheim Corp., New York, N. Y. 10017), neocuproine, and o-toluidine. Some of these procedures constitute a haz-ard because of carcinogenic reagents (1); some are suscepti-ble to interference from multiple nonsugar substances and from sugars other than glucose. These considerations prompted us to examine a modification (2) of the glucose oxidase (EC 1.1.3.4)/peroxidase (EC 1.11.1.7) method in which a different color reagent, 4-aminophenazone, is used. This compound is innocuous in normal laboratory use (2). Furthermore, uric acid, ascorbic acid, and other common interfering substances are said (3) not to interfere with the color reaction. Material and Methods We used the AutoAnalyzer I (Technicon Instrument