SUMMARY

Grapevines from six Chilean regions were surveyed for virus diseases and tested for the presence of the most important viruses. ELISA testing of 2535 samples and confirmatory RT-PCR of some ELISA-negative samples from symptomatic and symptomless vines gave the fol-lowing infection rates: 6.36 % for Grapevine fanleaf virus (GFLV); 4.67 % for Grapevine leafroll associated virus 1 (GLRaV-1); 16.05 % for Grapevine leafroll associated virus 2 (GLRaV-2); 6.41 % for Grapevine leafroll associat-ed virus 3 (GLRaV-3); 0.26 % for Grapevine leafroll asso-ciated virus 7 (GLRaV-7); 14.99 % for Grapevine fleck virus (GFkV); 5.57 % for Grapevine virus A (GVA) and 0.78 % for Grapevine virus B (GVB). Strawberry latent ringspot virus (SLRSV) Tomato ringspot virus (ToRSV) and Arabis mosaic virus (ArMV) were not detected. Overall infection in the surveyed Chilean grapevines, considering ELISA and RT-PCR, was 32.35%. Virus in-fection ratio obtained from ELISA analysis in the six re-gions, varied between 21.19 % (Región Metropolitana) and 74.26 % (Coquimbo). RT-PCR was used for detec-tion of the Red Globe strain of GLRaV-2 and Grapevine rupestris stem pitting associated virus (GRSPaV), and t