Add to ORKGBACKGROUND: Methods are needed to analyze small amounts of samples for variation in disease-causing genes. One means is to couple the sensitivity and mul-tiplexing capability of the ligation detection reaction (LDR) with the use of simple glass slides specifically functionalized with a novel polymer coating to en-hance sensitivity. METHODS: Wedeveloped an array-based genotyping as-say based on glass slides coated with copolymer (N,N-dimethylacrylamide, N,N-acryloyloxysuccinimide, and 3-(trimethoxysilyl)propyl methacrylate). The as-say consists of an LDR with genomic DNA followed by a universal PCR (U-PCR) of genomic DNA–templated LDR product. The LDR occurs in the presence of 3 primers for each sequence variant under investigation
<div><p>To simultaneously analyze mutations and expression levels of multiple genes on one detection...
Abstract Background Rolling circle amplification of ligated probes is a simple and sensitive means f...
BACKGROUND: Microelectronic DNA chip devices represent an emerging technology for genotyping. We dev...
BACKGROUND: Methods are needed to analyze small amounts of samples for variation in disease causing ...
We can detect the b-globin gene sickle cell mutation by using an assay based on the ligase chain rea...
The identification of sporadic point mutations in tumor-suppressor genes, which are responsible for ...
One major goal of genetic research is to understand the role of genetic variation. By far the most c...
sent an emerging technology for genotyping. We devel-oped methods for detection of single-nucleotide...
We present a novel microfluidic-based approach to detect ligation products. The conformal specificit...
With the identification of common single locus point mutations as risk factors for thrombophilia, ma...
cons with the DNA dye LCGreenTM I was recently introduced as a homogeneous, closed-tube method of ge...
with saturation dyes for either mutation scanning of PCR products or genotyping with unlabeled probe...
In this article, we describe a genotyping approach applicable to both individual and multiplexed sin...
The ability to analyze multiple polymorphic/mutation sites rapidly and accurately is pivotal in all ...
International audienceSuccessful use and reliability of microarray technology is highly dependent on...
<div><p>To simultaneously analyze mutations and expression levels of multiple genes on one detection...
Abstract Background Rolling circle amplification of ligated probes is a simple and sensitive means f...
BACKGROUND: Microelectronic DNA chip devices represent an emerging technology for genotyping. We dev...
BACKGROUND: Methods are needed to analyze small amounts of samples for variation in disease causing ...
We can detect the b-globin gene sickle cell mutation by using an assay based on the ligase chain rea...
The identification of sporadic point mutations in tumor-suppressor genes, which are responsible for ...
One major goal of genetic research is to understand the role of genetic variation. By far the most c...
sent an emerging technology for genotyping. We devel-oped methods for detection of single-nucleotide...
We present a novel microfluidic-based approach to detect ligation products. The conformal specificit...
With the identification of common single locus point mutations as risk factors for thrombophilia, ma...
cons with the DNA dye LCGreenTM I was recently introduced as a homogeneous, closed-tube method of ge...
with saturation dyes for either mutation scanning of PCR products or genotyping with unlabeled probe...
In this article, we describe a genotyping approach applicable to both individual and multiplexed sin...
The ability to analyze multiple polymorphic/mutation sites rapidly and accurately is pivotal in all ...
International audienceSuccessful use and reliability of microarray technology is highly dependent on...
<div><p>To simultaneously analyze mutations and expression levels of multiple genes on one detection...
Abstract Background Rolling circle amplification of ligated probes is a simple and sensitive means f...
BACKGROUND: Microelectronic DNA chip devices represent an emerging technology for genotyping. We dev...