CSF-1 receptor structure/function in MacCsf1r–/– macrophages: regulation of proliferation, differentiation, and morphology

Abstract: CSF-1 is the major regulator of tissue macrophage development and function. A GM-CSF-dependent, CSF-1 receptor (CSF-1R)-defi-cient F4/80hiMac-1Gr1–CD11c bone marrow macrophage (BMM) line (MacCsf1r/) was devel-oped to study the roles of the eight intracellular CSF-1R tyrosines phosphorylated upon receptor activation. Retroviral expression of the wild-type CSF-1R rescued the CSF-1-induced survival, pro-liferation, differentiation, and morphological char-acteristics of primary BMM. Mutation of all eight tyrosines failed to rescue, whereas the individual