Effective Regeneration of Impatiens Campanulata Wight a

Abstract: An in vitro plant regeneration protocol was successfully established in Impatiens campanulata, an important medicinal plant by tissue culture methods.The plants were raised and development of protocorm was highest in MSmedium. In the present investigation universal media MS, B and WPM were used. The5 composition of three different media is represented are very active for regeneration of Impatiens campanulata.The principal component of most tissue culture media can be categorized into inorganic nutrients (macro and micro), organic nutrients (vitamins and amino acids), carbon source, plant growth regulators and gelling agent.. To standardize a perfect protocol for In vitro rooting and subsequent acclimatization of plantlets of Impatiens campanulata. Key words: Impatiens campanulata Tissue culture Inorganic nutrients Regeneration INTRODUCTION and curative medicines aimed at new drug discoveries for Today over 95 % of the medicinal plants used by the Due to fast changing life style globally today the industries in India are collected from forests and other demand for plant based pharmaceuticals is continuously natural resources. Less than 20 plant species are collected on the rise. Though medicinal plants are of great under commercial cultivation, while over 400 species used commercial value most of them are still of wild origin