Monitoring Tyrosine Phosphorylation Capture of specific tyrosine phosphorylated

Src Homology 2 (SH2) domain-containing proteins are critical for mediating cellular signaling from receptor tyrosine kinases. These modular protein interaction domains function by recognizing and binding to specific phosphotyrosine residues present in many signaling proteins. These interactions are crucial for signal transmission from receptor tyrosine kinases to downstream targets, resulting in regulation of multiple biological processes. The new Thermo Scientific SH2 Phosphotyrosine Capture Kits enable researchers to monitor specific tyrosine phosphorylation events and identify SH2 domain protein-protein interaction networks. Each kit contains the necessary reagents including negative controls to perform six pull-down reactions from mammalian cell lysates. Analysis of SH2 domain interactions may be performed by Western blot or mass spectrometry. Highlights