Microsatellite Isolation and Linkage Group Identification in the Yellow Fever Mosquito Aedes aegypti

Microsatellites have proved to be very useful as genetic markers, as they seem to be ubiquitous and randomly distributed throughout most eukaryote genomes. However, our laboratories and others have determined that this paradigm does not necessarily apply to the yellow fever mosquitoAedes aegypti. We report the isolation and identification of microsatellite sequen-ces from multiple genomic libraries for A. aegypti. We identified 6 single-copy simple microsatellites from 3 plasmid libraries enriched for (GA)n, (AAT)n, and (TAGA)n motifs fromA. aegypti. In addition, we identified 5 single-copy microsatellites from an A. aegypti cosmid library. Genetic map positions were determined for 8 microsatellite loci. These markers greatly increase the number of microsatellite markers available for A. aegypti and provide additional tools for studying genetic variability of mosquito populations. Additionally, most A. aegypti microsatellites are closely associated with repetitive elements that likely accounts for the limited success in developing an extensive panel of microsatellite marker loci. Microsatellites or simple sequence repeats are short stretches of DNA sequence in which motifs of 1–6 bases are tandemly repeated (Weber and May 1989; Schlötterer 2000). They have become the genetic marker of choice for many eukaryotic species because of their high variability, codominant expres