Effect of Defaunation and Supplementation Methionine Hydroxy Analogue and Branched Chain Amino Acid in Growing Sheep Diet Based on

Abstract: This experiment tried to make use the ample supply of palm press fiber (PPF) as the source of roughage. The experiment used in 5x5 Latin Square feeding trial in sheep of 9.4±1.63 kg live weight. The experiment diets composed of 50 % elephant grass or 50 % PPF and 50 % concentrate. The treatment were A = 505 % elephant grass, B = 50 % PPF, previously treated with 1.5 % urea, C = B+1.5 % corn oil, D = C+0.1% Ca salt of methionine hydroxy analogue (MHA) and E = D+0.1 % val+0.15 % leu dan 0.2 % ile (Branced Chain Amino Acid = BCAA). Animal on the ammoniated PPF diet (B) had less cultivable rumen bacteria than those on elephant grass 90.88 x 10 vs 1.09 x 10 colonies/ml). Addition of corn oil (C) reduced the viable rumen11 11 protozoa (1.43 x 10 to 1.27 x 10 cell c/ml. The decrease was accompanied by the increase in bacteria to5 5 1.14 x 10 colonies/ml and digestibility to 59.4%. Supplementation of MHA (D) increased the bacteria to11 1.63 x 10 colonies/ml. The bacterial counts were increased further to 1.89 x 10 colinies/ml by the BCAA (E).11 The later treatment was better in digestibility and N retention than the control but had same effect on growth performance of the animal (104 vs 102 g/d). The live weight gain of the later treatment (E) was significantly better than those on treatment B, C or D. Key words: Palm press fiber, defaunation, MHA, BCC