Muscle type-specific response of HSP60, HSP72, and HSC73 during recovery after elevation of muscle temperature

2001.—An original method to induce heat stress was used to clarify the time course of changes in heat shock proteins (HSPs) in rat skeletal muscles during recovery after a single bout of heat stress. One hindlimb was inserted into a stain-less steel can and directly heated by raising the air temper-ature inside the can via a flexible heater twisted around the steel can. Muscle temperature was increased gradually and maintained at 42°C for 60 min. Core rectal and contralateral muscle temperatures were increased 1.5°C during the heat stress. HSP60, HSP72, and heat shock cognate (HSC) 73 content in the slow soleus and fast plantaris in both limbs were determined immediately (0 h) and 2, 4, 8, 12, 24, 36, 48, or 60 h after heat stress. Within 0–4 h, all HSPs were 1.5-to 2.2-fold higher in heat-stressed than contralateral soleus. Compared with the contralateral plantaris, the heat-stresse