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Abstract — Accurate iris segmentation is presented in this paper, which is composed of two parts, reflection detection and eyelash detection. Eyelashes are classified into two categories, separable and multiple. An edge detector is applied to detect separable eyelashes and intensity variances are used to recognize multiple eyelashes. Reflection is also divided into two types, strong and weak. A threshold and statistical test are proposed to recognize the strong and weak reflection, respectively. We have developed an iris recognition approach for testing the effectiveness of the proposed segmentation method. The results show that the proposed method can reduce recognition error for the iris recognition approach