ANTAGONISTIC EFFECT OF STAPHYLOCOCCUS HYICUS AND STAPHYLOCOCCUS CHROMOGENES.EXlUBlTED ON STAPHYLO- COCCAL DELTA HEMOLYSIN

Abstract. Skalka B.: Antagonistic Effect of Staphylococcus hyicus and Staphylococcus chromo-genes Exhibited on Staphylococcal Delta Hemolysin. Acta vet. Bmo, 60,1991: 61-69. Staphylococcus hyicus and Staphylococcus chromo genes are able to inhibit hemolytic effect of the staphylococcal delta hemolysin on agar media supplemented with human or sheep erythrocytes. This inhibition takes place even when the effect of delta hemo-lysin on agars with sheep erythrocytes is potentiated with staphylococcal beta hemo-lysin. Although less markedly expressed, similar antagonistic effect on staphylococcal delta hemolysin is also found in ' a majority of strains Staphylococcus sciuri, Staphylo-coccus lentus and some Micrococcus spp. Other species of coagulase-negative staphy-lococci have no antagonistic effect on delta hemolysin. A technique of a combined test of hemolytic interactions (CTHI) for a simultaneous assay of hemolytic antagonism and synergism was proposed, which can be used to differentiate S. hyicus and S. chro-mogenes from other novobiocin-sensitive staphylococcal species. Staphylococcus hyicus, Staphylococcus chromo genes, staphylococcal delta hemolysin, anta-gonistic effect, combined test of hemolytic interactions (CTHI) In our previous paper (Skalka 1988) we described hemolytic activity of S. hyicus and S. chro-:mogenes on blood agars with rabbit or human erythrocytes. Hemolytic effects of these two staphy-lococcal species on agar media supplemented with erythrocytes of various animal origin differed from the data on sensitivity of various erythrocytes to staphylococcal hemolysins alpha, beta, ga