Differential display (DD) is a powerful molecular tool that allows the identification and subsequent isolation of transcripts dif-ferentially expressed between biological samples, for example, between undifferenti-ated and differentiated cells, between differ-ent tissues or in one tissue at different stages of development. However, significantly high rates of ap-parent false positives have been reported using this technique. We suggest that the vast majority of false positives do not repre-sent the originally selected transcript, but instead result from the re-amplification of cDNA species that co-migrate with the cDNA of interest in DD gels. Here we describe the use of a procedure to resolve co-migrating cDNAs and to puri-fy the candidate ...
Recently, a novel PCR-based technique, differential display (DD), has facilitated the study of diffe...
Differential display PCR (DD-PCR) is an mRNA fingerprinting technique to identify differentially exp...
Alternative splicing, alternative transcript initiation, and alternative 3’ polyadenylation are the ...
The differential display by PCR (DD-PCR) technique (1,2) was conceived to allow the identification a...
abundance mRNAs Abstract: The high percentage of false positives generated by differential display (...
The high percentage of false positives generated by differential display (as high as 85%) has previo...
A solid-phase differential display method was designed to analyze differential gene expression in sa...
Differential display (DD) is a novel PCR-based technique, very commonly used to study differentially...
Since its conception in 1992, differential display PCR (DD-PCR) has attracted widespread interest. T...
This thesis describes an investigation of differential gene expression, comparing dissected somites ...
Representational difference analysis (RDA) is a powerful technique for cloning the differences betwe...
We have significantly improved a method originally developed by Liang and Pardee [Science 257 (1992)...
Summary. The technique of differential display (DD) has been used widely to identify potentially int...
Abstract Background Gene expression studies on non-model organisms require open-end strategies for t...
A reverse Northern analysis that effectively eliminates the false positives isolated from differenti...
Recently, a novel PCR-based technique, differential display (DD), has facilitated the study of diffe...
Differential display PCR (DD-PCR) is an mRNA fingerprinting technique to identify differentially exp...
Alternative splicing, alternative transcript initiation, and alternative 3’ polyadenylation are the ...
The differential display by PCR (DD-PCR) technique (1,2) was conceived to allow the identification a...
abundance mRNAs Abstract: The high percentage of false positives generated by differential display (...
The high percentage of false positives generated by differential display (as high as 85%) has previo...
A solid-phase differential display method was designed to analyze differential gene expression in sa...
Differential display (DD) is a novel PCR-based technique, very commonly used to study differentially...
Since its conception in 1992, differential display PCR (DD-PCR) has attracted widespread interest. T...
This thesis describes an investigation of differential gene expression, comparing dissected somites ...
Representational difference analysis (RDA) is a powerful technique for cloning the differences betwe...
We have significantly improved a method originally developed by Liang and Pardee [Science 257 (1992)...
Summary. The technique of differential display (DD) has been used widely to identify potentially int...
Abstract Background Gene expression studies on non-model organisms require open-end strategies for t...
A reverse Northern analysis that effectively eliminates the false positives isolated from differenti...
Recently, a novel PCR-based technique, differential display (DD), has facilitated the study of diffe...
Differential display PCR (DD-PCR) is an mRNA fingerprinting technique to identify differentially exp...
Alternative splicing, alternative transcript initiation, and alternative 3’ polyadenylation are the ...