ANALYSIS OF TIME FILTERING OF EXCITATION LIGHT IN FLUORESCENCE MEASUREMENT WITH SIMULATION MODELLING

This paper presents a simulation model of a typical fluorescence measurement setup. The model includes the simulation of light source, basic fluorescent sample, single-photon avalanche diode detector, read-out electronics and setup geometry. In this paper we employ our model to investigate the possibility of using time filtering instead of conventional optical filtering in fluorescence detection. In order to achieve this, we have performed a number of simulations of time-resolved fluorescence measurements with time filtering of the excitation light. The light source was simulated as a micro-LED array, the fluorescent sample as a solution of CdSe/ZnS quantum dots in toluene, and the detector as a CMOS SPAD array. The detected fluorescence was processed with time-correlated single photon counting technique