Review ^OTCCmOLCX:Y Atlantic Cod Trypsins: From Basic Research to Practical Applications

Atlantic cod trypsin I is an appropriate representa tive of the traditionally classified cold-adapted group I trypsins, and the recombinant form of cod trypsin Y is the only biochemically characterized member of the novel group III trypsins. Trypsin Y is adapted to lower temperatures than all other presently known trypsins. This review describes the basic character istics of and practical uses for trypsins of Atlantic cod, as well as those of other organisms. Overex pression of the recombinant forms of cod trypsins I and Y in microorganisms is explained as well as the advantages of using site-directed mutagenesis to in crease their stability toward autolysis and thermal inactivation. Trypsins appear to play a key role in the nutrition and development of marine fish. We discuss the potential use of cod trypsins as biomar kers to evaluate the nutritional status of cod larvae and describe the industrial applications of cod tryp sin I and other trypsins. Key words: trypsins — fish — cold-adapted — expression — practical application