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natu trea ork that al (B stra ch. Real-time quantitative PCR (qPCR) has become a definitive tech-nique for quantitating differences in gene tween samples. Over the past 10 years, method has grown exponentially, with the 25,000 papers from diverse fields of science environmental, industrial, and medical rese to RT (reverse transcription)-qPCR data. as stim om jou typic o minology, guidelines on minimum information for biological and handling methods within each group is essential to minimize var-iability (Table 1). Each of these parameters should be carefully re-corded prior to conducting gene expression experiments to assure good biological reproducibility for published data. 3. RNA extraction If samples must be collected over a period of time or in too large a number to process immediately, they should be stored in appro-q Information of this paper is corresponding to our currently written tech not