Add to ORKGWe present a method for the in vitro am-plification of>6.0 kb of DNA flanking a known site. This is accomplished by ligating an oligonucleotide to create an inverted re-peat of a portion of the known sequence, followed by single-primer polymerase chain reaction (PCR) amplifications. This method generates a panhandle template following primer extension on the strand of interest. It does not involve template-directed exten-sion from the ligated oligonucleotide, and it is carried out without DNA extractions. We have used this method to amplify 4.5–9.4 kb of DNA flanking the original primer an-nealing sites directly from human genomic DNA
method that allows the researcher to generate a representative amplification of genomic DNA. The kit...
As Sanger sequencing is being replaced by higher throughput and lower cost of next generation sequen...
Several methods for site-directed mutagenesis using PCR have been described in the last few years. O...
We describe a simple PCR-based method for the isolation of genomic DNA that lies adjacent to a known...
International audienceA simple and efficient ligation-mediated PCR (LMPCR) is described for amplifyi...
A method for the amplification of a single DNA strand at Dow copy number is described. U is a wholly...
Several whole genome amplification strategies have been developed to preamplify the entire genome fr...
High levels of non-authentic sequence data can be generated by traditional PCR-based methodologies w...
Site-directed mutagenesis is an invaluable tool for functional studies and genetic engineering. Howe...
The products of polymerase chain reaction (PCR) (1) are usually visualized by agarose or polyacrylam...
We describe a simple PCR-based method for the isolation of genomic DNA that lies adjacent to a known...
A simple and efficient method is described for the isolation of extension fragments of known DNA seq...
In this study we present an improved polymerase chain reaction (PCR)-based methodology to generate l...
We describe a new rapid and efficient polymerase chain reaction (PCR)-based site-directed mutagenesi...
A method is described for the absolute quantification by polymerase chain reaction (PCR) of nucleic ...
method that allows the researcher to generate a representative amplification of genomic DNA. The kit...
As Sanger sequencing is being replaced by higher throughput and lower cost of next generation sequen...
Several methods for site-directed mutagenesis using PCR have been described in the last few years. O...
We describe a simple PCR-based method for the isolation of genomic DNA that lies adjacent to a known...
International audienceA simple and efficient ligation-mediated PCR (LMPCR) is described for amplifyi...
A method for the amplification of a single DNA strand at Dow copy number is described. U is a wholly...
Several whole genome amplification strategies have been developed to preamplify the entire genome fr...
High levels of non-authentic sequence data can be generated by traditional PCR-based methodologies w...
Site-directed mutagenesis is an invaluable tool for functional studies and genetic engineering. Howe...
The products of polymerase chain reaction (PCR) (1) are usually visualized by agarose or polyacrylam...
We describe a simple PCR-based method for the isolation of genomic DNA that lies adjacent to a known...
A simple and efficient method is described for the isolation of extension fragments of known DNA seq...
In this study we present an improved polymerase chain reaction (PCR)-based methodology to generate l...
We describe a new rapid and efficient polymerase chain reaction (PCR)-based site-directed mutagenesi...
A method is described for the absolute quantification by polymerase chain reaction (PCR) of nucleic ...
method that allows the researcher to generate a representative amplification of genomic DNA. The kit...
As Sanger sequencing is being replaced by higher throughput and lower cost of next generation sequen...
Several methods for site-directed mutagenesis using PCR have been described in the last few years. O...