Effect of ovariectomy on biochemical markers of bone turnover (ALP, ACP) and calcium content in rat mandible and teeth. Bull Vet Inst Pulawy

The aim of this study was to determine the correlation between the level of calcium in the mandible and teeth and the level of alkaline and acid phosphatases in ovariectomized rats with or without supplementation of 17β-estradiol. Rats were qualified for the following groups: Cl – control, SH – sham- operated, OV – ovariectomized, OVH – ovariectomized and receiving 17β-estradiol in three different doses (1.25, 12.5, 125 μg) during seven weeks. The negative correlation between level of calcium in the examined tissue and the level of both phosphatases was observed. Key words: rat, bones ovariectomy, 17β-estradiol, alkaline phosphatase, acid phosphatase. Bone metabolic processes are mainly regulated by hormonal milieu. It can influence the activity of a group of enzymes. Changes in the bone tissue can be also activated by bone turnover markers. They have been divided into markers of bone resorption and bone formation (15). Among the bone formation markers we can distinguish serum concentration of alkaline phosphatase (ALP), osteocalcin (OC) and type I collagen. Markers of bone resorption are: hydroxyproline (OHP), hydroxylysin